Please email the required application materials to Stephanie Lettieri at email@example.com
Postdoctoral Fellow Position
The Mignot Laboratory has an opening for a highly motivated, self-driven postdoctoral fellow with a background in cellular and molecular immunology. The successful candidate will join Stanford University School of Medicine.
The aim of this project is to understand how flu vaccinations or infections trigger or protect against narcolepsy, an autoimmune disease caused by a loss of hypocretin neurons. To address this question, we are characterizing T cell responses to H1N1 Influenza strain and hypocretin peptides that are involved in the development of narcolepsy, comparing responses in HLA-DQ*06:02 positive narcoleptics and healthy individuals.
The project is at a very exciting juncture. A specific hypocretin epitope that is post-translationally modified has been identified as important for disease causation. We also found a specific H1N1 HA sequence homologous to the autoimmune hypocretin epitope and identified specific crossreactive T cell receptors through tetramer studies, suggesting molecular mimicry. Genetic studies of narcolepsy suggest causality of these mechanisms based on associations with genetic factors that modulate usage of V/J TCRT segments involved in the TCR identified.
The approach is based on
1- The identification of flu antigens from H1N1 that could cross-react with hypocretin when presented by DQ0602.
2- The identification of antigen-specific cells using DQ0602 tetramers
3- The single-cell TCR sequencing of antigen-specific T cells.
4- The phenotypic and functional characterization of culprit antigen-specific T cells using flow cytometry, droplets and CyTOF
- The selected postdoc candidate will have a PhD or MD/PhD
- Knowledge in experimental skills including cell culture, flow cytometry, sequencing and a background in human cellular and molecular immunology
- Demonstrated ability to work independently, self-initiative, attention to details, be dedicated, and self-motivated with a strong aptitude for team work
- An updated CV
- Complete contact information for three references
- A cover letter describing their past research experience
- Career goal and a statement of future research interest (1-2 pages)