Posted on Thu, 09/05/2024 - 13:59

Important Info

Faculty Sponsor (Last, First Name): 
Tolias. Andreas
Stanford Departments and Centers: 
Ophthalmology
Appointment Start Date: 
Immediately available
Group or Departmental Website: 
http://toliaslab.org
How to Submit Application Materials: 

Please send your CV and one page interest statement to: camilal@stanford.edu and ammck@stanford.edu

Does this position pay above the required minimum?: 
No. The expected base pay for this position is the Stanford University required minimum for all postdoctoral scholars appointed through the Office of Postdoctoral Affairs. The FY25 minimum is $73,800.

SUMMARY

The specific duties of the postdoctoral research fellow will be to use whole-cell patching methods to probe the cellular diversity of brain circuits across cortical areas and species at the level of morphology, electrophysiology and transcriptome.


Description 

The laboratory of Dr. Andreas Tolias in the Department of Ophthalmology at Stanford University School of Medicine is seeking applicants for a postdoctoral research fellowship. Applicants will work collaboratively within a multi-disciplinary research team aimed at understanding the cellular diversity of brain circuits. The specific goals of these positions will be to use whole-cell patching methods, single-cell RNA-sequencing and immunochemistry to probe morphology, electrophysiology and transcriptome of the individual neurons of brain circuits across cortical areas and species including humans. In particular, Patch-seq, a method we recently developed, that combines whole-cell patch-clamp recordings and single-cell RNA sequencing (scRNA-seq). The fellow will receive extensive one-on-one training with postdocs and junior faculty on cellular electrophysiology to develop the necessary skills to acquire high-quality data. There will also  opportunity of learn multi-electrode recordings using Neuropixels and advance their computational and programming skills. 

The ideal applicants should have some experience in cellular electrophysiology, PCR or RNA-sequencing. The candidates without these experiences will be also considered if they are willing to commit the efforts to develop these skills. 

You can learn more about our research at http://toliaslab.org and more specific information about the current project in recent publications from our lab:

Scala, F., et al. (2021). "Phenotypic variation of transcriptomic cell types in mouse motor cortex." Nature 598(7879): 144-150.

Cadwell, C. R., et al. (2017). "Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using Patch-seq." Nat Protoc 12(12): 2531-2553.

Jiang, X., et al. (2015). "Principles of connectivity among morphologically defined cell types in adult neocortex." Science 350(6264): aac9462.

Required Qualifications: 

Required: PhD in a basic science or equivalent education. 

Preferred: PhD in a neuroscience or related degree.

 

Experience: 

Experience with patch-clamp is preferred. Two years of directly related experience is preferred.

Required Application Materials: 
  • CV 
  • one page Cover Letter Interest Statement

 

Stanford is an equal opportunity employer and all qualified applicants will receive consideration without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, veteran status, or any other characteristic protected by law.