Department: DPB – Plant Biology Xu lab and Stanford University Walbot lab
Salary: Stanford scale
A Postdoctoral Fellow position is available to analyze maize anther proteomes, jointly sponsored by Dr. Virginia Walbot (Stanford University) and Dr. Shouling Xu (Department of Plant Biology, Carnegie Institution for Science. Using fully fertile and male-sterile, anther defective mutants already characterized by microscopy and single cell RNA-seq, learn diverse proteomics techniques to monitor protein abundances, de novo synthesis, and post-translational modifications in developing anthers to answer fundamental questions in developmental biology. Excellent training opportunity to learn cutting edge mass spectrometry and proteome analysis tools applied to a fundamental problem.
Nan, G-L., et al. 2022. Cascades of bHLH-regulated pathways program maize anther development. Plant Cell 34: 1207-1225. doi: org/10.1093/plcell/koac007 Highlight M. Osnato. 2022. Fantastic four: bHLH factors and the making of pollen. Plant Cell 34: 1151-1152. doi.org/10.1093/plcell/koac028
Nelms, B. and V. Walbot. 2022. Gametophyte genome activation occurs at pollen mitosis I in maize. Science 375: 284-289, plus the cover. doi: 10.1126/science.abl7392
Bi, Y. et al. 2021. Arabidopsis ACINUS is O-glycosylated and regulates transcription and alternative splicing of regulators of reproductive transitions. 2021. Nat Commun.12: 945. doi.org/10.1038/s41467-021-20929-7
Reyes, A. V. et al. 2022. Application of parallel reaction monitoring in 15N labeled samples for quantification. Front. Plant Sci., doi.org/10.3389/fpls.2022.832585
Candidates will perform MS work at Carnegie Mass Spec facility (on the Stanford campus) directed by Dr. Shouling Xu.
The facility is currently equipped with Orbitrap Q-Exactive and is expected to have additional Orbitrap Eclipse with ETD in the fall. The facility is equipped with three HPLC/FPLC instruments for offline fractionations.
- Doctoral degree
- We prefer candidates with experience in either maize genetics/molecular biology or proteomics to work with the Walbot lab on carefully staged anthers and specific anther cell types.
- Cover letter
- Names of 3 references