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Perform and direct advanced genetic, genome engineering and stem cell research related to developmental biology and retinal disease. Also supervise members in the laboratory and organization.
BASIC FUNCTION AND RESPONSIBILITY:
Perform highly technical and specialized scientific research by planning research experiments, analyzing data and disseminating research findings. Key areas of responsibility include helping PhD candidates and medical students plan experiments, creating mouse models for genetic disorders; maintaining and studying mouse and human cell lines; analyzing and evaluating data/research; disseminating research results; writing of research manuscripts, proposals/grants; and selecting companies as sources of cutting-edge reagents and services.
KEY AREAS OF RESPONSIBILITIES:
• Work independently on research tasks with limited oversight by Principal Investigator (PI).
• Establish and maintain cultures of human cells as well as the ability to differentiate human cell lines into retinal tissue.
• Assist in the development and execution of experimental protocols designed to study genetic mutations found in developmental disorders and retinal disease.
• Perform transfections and genome engineering experiments on both mouse and human cell lines.
• Inject and/or transplant human cells/viruses into mouse models.
• Mouse handling, breeding, genetics, and surgical experience with the retina is required.
• Investigate pathophysiological mechanisms of photoreceptor degeneration associated with vitreoretinopathies using cell culture systems and rodent models and elecroretinography.
• Plan, perform, and analyze results from in vitro and in vivo experiments on an as needed basis. This will include: extracellular tissue analysis, embryo dissection, microscopy (fluorescence, confocal, and lightsheet), immunohistochemistry, southern blot analysis, RT-PCR, qPCR, and western blotting. This may also include: flow cytometry and enzymatic assays.
• Collect and analyze experimental data and evaluate relevance of the data.
• Develop hypotheses to be tested and methodology for evaluating results.
• Compare experimental data with previously published work and develop and test new hypotheses.
• Supervise, train and collaborate with graduate students and other members of the laboratory, and conduct continuing education.
• Manage all or part of a research project including staff selection, assignment and scheduling, monitoring of project funds, coordination of data generation and analysis and identification, acquisition and use of equipment and facilities.
• Participate in determining research priorities and planning programs of research.
Dissemination of Research Results
• Prepare manuscripts for publication.
• Present results in departmental and conference seminars.
• Independently prepare to new or continuing research grant proposals while working within a scientific writing team.
• Contribute to progress reports required by funding agencies.
• Supervise adherence to safety, lab managerial tasks, and compliance guidelines when necessary.
• Inspires and motivates others to high performance by exercising strong stewardship of University resources, setting expectations, measuring success through individual performance evaluations, and driving organizational results.
• Participate in the development of goals for the research project and in the planning of laboratory work; plan and coordinate the work of graduate students and other support personnel; and serve as a consultant to the project director, faculty and staff within the department. Instruct lab personnel in proper procedures and techniques.
- A Ph.D. in a field pertaining to stem cell biology, genetics, and ophthalmology, or equivalent combination of education and experience.
- Experience in a field pertaining to stem cell biology and/or genome engineering, or equivalent combination of education and experience.
- Strong publication record in peer-reviewed journals.
- Experience with lentivirus and AAV development.
- Expertise in surgical procedures such as, but not limited to, subretinal injections, intravitreal injections, in utero injections, and cell transplantation.
- Expertise in human cell culture and genome engineering technologies such as CRISPR/Cas9, TALENs, or ZFNs
- Expertise in intra- and inter-species chimeric research (incorporating primary donor cells, as well as stem cell-derived donor cells during mouse development)
- The candidate should be proficient in standard molecular biology techniques (e.g. cloning, site-directed mutagenesis, and RT-PCR, DNA and RNA isolation, quantitative Real Time PCR), common biochemical techniques (e.g. expression and purification of recombinant and tagged protein, protein isolation and purification from human and mouse tissue samples), common cell biology techniques (e.g. immunofluorescence) and tissue culture techniques (i.e. establishing and maintaining primary cell lines and generating stable cell lines).
- Excellent oral, written, and interpersonal skills are required. The candidate must be able to integrate into a highly cooperative research team and take direction from the PI and supervising research staff designated by the PI.
- Experience writing NIH grants.
- Experience with mouse eye electroretinography and mouse eye imaging (fundus photography, optical coherence tomography, auto-fluorescent imaging, and infrared imaging) and phenotyping.
- Experience teaching and training undergraduate and graduate students.
- Nutritional metabolism.
- Demonstrated experience with Photoshop and Macintosh software.
- Experience speaking in professional settings (annual meetings, department seminars, etc.).
- Ability to prepare surgical equipment, such as specialized injection needles
- Cover letter